![]() ![]() Received: OctoAccepted: JanuPublished: February 18, 2020Ĭopyright: © 2020 Kovaltsuk et al. PLoS Comput Biol 16(2):Įditor: Yanay Ofran, Bar Ilan University, ISRAEL (2020) Structural diversity of B-cell receptor repertoires along the B-cell differentiation axis in humans and mice. Finally, we established the differences in CDR usages between humans and mice, analysis with immediate relevance for BCR repertoire “humanness” assessment and rational immunotherapeutic engineering.Ĭitation: Kovaltsuk A, Raybould MIJ, Wong WK, Marks C, Kelm S, Snowden J, et al. Our results suggest that increased B-cell differentiation is associated with a personalization of CDR structure usages. We showed that naïve repertoires share the highest number and diversity of CDR structures, a pattern which was highly conserved in all B-cell donors. By analysing human and mouse BCR repertoires at an unprecedented scale, we observed species-specific structural predetermination and detected CDR dynamics across multiple stages of B-cell differentiation. Here we report our novel rapid pipeline, SAAB+, which has enabled us to interrogate how the structure of the CDR changes in BCR repertoires along the B-cell differentiation axis. Drug discovery and immunodiagnostics inspired by the adaptive immune system rely on our ability to accurately interrogate the structural diversity of the binding sites of the BCR repertoire. Each individual has a huge BCR repertoire, where each individual BCR has a specific binding site composed of the complementary-determining regions (CDRs) capable of recognising a specific antigen. These are immunoglobulin molecules that bind to foreign substances known as antigens. ī-cell receptors (BCR) are the major components of the adaptive immune system. The software tool for structural annotation of BCR repertoires, SAAB+, is available at. ![]() Our results establish the CDR structure differences in BCR repertoires and have applications for many fields including immunodiagnostics, phage display library generation, and “humanness” assessment of BCR repertoires from transgenic animals. In contrast, more differentiated B-cells are more personalized in terms of CDR structure usage. Antigen-unexperienced BCR repertoires use the highest number and diversity of CDR structures and these patterns of naïve repertoire paratope usage are highly conserved across subjects. ![]() We show that B-cell types can be distinguished based solely on these structural properties. These structurally annotated CDRs provide unprecedented insights into both the structural predetermination and dynamics of the adaptive immune response. We have used novel rapid methods to structurally characterize the complementary-determining regions (CDRs) of more than 180 million human and mouse B-cell receptor (BCR) repertoire sequences. Most current analysis tools for antibody next-generation sequencing data work with primary sequence descriptors, leaving accompanying structural information unharnessed. ![]()
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